Abstract: Direct Linear Transformation (DLT) is one of main algorithms used in 3D motion image analysis, moreover the spatial coordinates of control points are the fundamental parameters to calibrate the cameras using DLT algorithm, and are usually given by 3D calibration frame.Because of the limited room covered by 3D calibration frame, the error will occur when using 3D motion image analysis system to track and measure the moving object in the relative large range.The purpose of this study was to develop the software system to measure control points and transform coordinates using C# programming language with the total station (Topcon GPT-3002N) .This system can provide an applied tool to survey the accurate spatial coordinates of control points positioned as you wish in the large range, or fixed on the 3D calibration frame, and meet the needs to carry out 3D tracking motion image analysis in analyzing and diagnosing the techniques in sport movements.In addition, the demands of the system to measure control points and transform coordinates, general structure, design of database and algorithm, functions and realization in practice were describe in details in this paper.