Abstract: Purpose:To evaluate the effect of high-top and low-top basketball shoes on ankle inversion kinematics, ground reaction forces and relevant muscle activities, as well as the relationship between shoes and ankle stability during landing.Methods:13 subjects were required to wear high-top and low-top basketball shoes to land on an inversion & plantarflexion tilting platform.Kinematics, ground reaction force and corresponding muscles activities of ankle were collected and analyzed.Result:No significant effect was found in inversion angle, maximum inversion angle velocity, and ground reaction force for vertical & horizontal directions.However, high-top shoe showed a later onset of pre-activation than low-top shoe in four tilting conditions, especially at a 15° inversion condition (P<0.05) ;meanwhile, the amplitude of pre-activation of peroneus longus and peroneus brevis for high-top shoe were smaller than low-top shoe at 15° inversion condition and 25° inversion plus 20° plantarflexion condition, respectively (P<0.05) .Conclusions:The high-top shoe selected in this study has little effects on ankle kinematics and ground reaction force, but has certain positive effects on ankle muscle pre-activation and limitation of range of motion.