Abstract: Background and Objective:Low intensity monochromatic light(LIL) therapy was widely applied in athletic training.The LIL modulation of high glucose(HG) induced myoblast stress and its mechanism was studied in this paper.Study Design/Materials and Methods:C2C12 myoblasts were cultivated in glucose at different concentrations including 22.5(NG) and 90.0(HG) mmol/L and then irradiated with red light at 640±15 nm from light-emitting diode array(RLED) at I mW/cm2 for 15 min once a day for N days(I*N)(I=0.035,0.067;N=1,2,3),respectively.They were also pretreated with resveratrol(RES) at 1-100 μmol/L for M h(RES1-100/M)(M=6,12,24) in NG and then cultivated at NG/HG for 3 days.The cellular parameters were routinely assessed.Results:1)The proliferation peaked in NG.2)0.035*3 did not modulate the proliferation in NG,but I*N(I=0.035,0.067;N=1,2,3) significantly improved the proliferation in HG on the 3rd and 4th day,respectively.3)RES5-100/6,RES1-100/12 and RES1-30/24 significantly promoted the proliferation in HG,and moreover,RES10/12 significantly promoted the proliferation in NG.4)0.035*3 significantly increased the mRNA expression of sirtuin 1(SIRT1),manganese superoxide dismutase(MnSOD) and muscular insulin-like growth factor-1(mIGF-1),but significantly decreased the one of forkhead class O 3a(FOXO3a),p27 and Bcl-2 interacting mediator of cell death(BIM).RES10/12 significantly increased SIRT1 mRNA expression,significantly decreased p27 mRNA expression,but did not modulate the mRNA expression of FOXO3a and BIM.5)HG significantly increased at the 4th h but decreased at the 72nd h the ratio of nicotinamide adenine dinucleotide(NAD+) and its reduced form NADH and SIRT1,which were recovered by 0.035*1 and 0.035*3,respectively.Conclusions:The proliferation of C2C12 myoblasts may be in proliferation-specific homeostasis in glucose at concentrations 22.5 mmol/L group,in which the proliferation may not be modulated by red light,but may be promoted by resveratrol.The establishment of proliferation-specific homeostasis in glucose at concentrations 90.0 mmol/L group may be promoted by red light through mIGF-1/SIRT1/FOXO3a pathways.