Abstract: Objective:To analyze the fatigue diagnosis of athletes by various methods in different training cycle.Methods:12 elite athletes from Beijing Rowing Team were involved in the study,using the blood biochemical indexes,psychological questionnaires and Omega Wave system to evaluate athletes's fatigue state in different training cycle.Results:Serum creatine kinase of athletes in the high load intensity training-week was significantly higher than the basic load training-week(P<0.05);The score of high load quantity training-week of health and fatigue scale was significantly lower than the basic load training-week(P<0.05);Activity of sympathetic regulation mechanisms of athletes in the high load quantity training-week was significantly lower than the basic load training-week(P<0.05);Stress index of athletes in the high load quantity training-week was significantly higher than the basic load training-week(P<0.05);Conclusion:The sustained and high load quantity training could lead to psychological or physical fatigue of athletes;Omega Wave system has active effect in early detection physiological fatigue of athletes.