张前锋, 徐晓阳, 李捷, 方方, 马涛. 2014: 不同训练方案对大鼠股直肌线粒体呼吸链复合体酶活性的影响. 体育科学, 34(10): 67-71. DOI: 10.16469/j.css.2014.10.009
    引用本文: 张前锋, 徐晓阳, 李捷, 方方, 马涛. 2014: 不同训练方案对大鼠股直肌线粒体呼吸链复合体酶活性的影响. 体育科学, 34(10): 67-71. DOI: 10.16469/j.css.2014.10.009
    ZHANG Qian-feng, XU Xiao-yang, LI Jie, FANG Fang, MA Tao. 2014: Effects of Different Straining Schemes on the Activities of Mitochondrial Respiratory Chain Complexes in Rat Rectus Muscles. China Sport Science, 34(10): 67-71. DOI: 10.16469/j.css.2014.10.009
    Citation: ZHANG Qian-feng, XU Xiao-yang, LI Jie, FANG Fang, MA Tao. 2014: Effects of Different Straining Schemes on the Activities of Mitochondrial Respiratory Chain Complexes in Rat Rectus Muscles. China Sport Science, 34(10): 67-71. DOI: 10.16469/j.css.2014.10.009

    不同训练方案对大鼠股直肌线粒体呼吸链复合体酶活性的影响

    Effects of Different Straining Schemes on the Activities of Mitochondrial Respiratory Chain Complexes in Rat Rectus Muscles

    • 摘要: 目的:研究不同训练方案运动后大鼠静息状态线粒体的呼吸链复合酶活性的特异性、适应性变化, 对不同训练方案的训练效果及其机理进行探索;为尝试寻找更有效的专项训练方法提供一些科学依据和参考。方法:将86只SPF级雄性SD大鼠随机分为对照组 (S) 、耐力组 (E) 、间歇组 (I) 、耐力+间歇组 (EI) 及间歇+耐力组 (IE) , 于4周、6周、8周运动结束48h后取样, 提取左侧股直肌线粒体, 测定线粒体呼吸链复合体酶Ⅰ、Ⅳ活性。结果:4周和6周运动后, 唯有E6组大鼠线粒体呼吸链复合体酶IV活性显著高于S6组;8周运动后, 复合体酶I活性I8组和IE8组显著高于S8组;复合体酶IV活性EI8组和I8组均显著高于S8组, IE8组极显著高于S8组。结论:4种训练方案均能使大鼠两种线粒体呼吸链复合酶活性提高, 其中, 耐力训练能最快使线粒体呼吸链产生适应性变化;间歇训练显著提高无氧代谢水平, 但线粒体呼吸链的适应较慢;间歇+耐力训练可能最有利于快速提高机体有氧代谢水平。

       

      Abstract: Objective:To study the specific and adaptive changes of mitochondrial respiratory chain complexes in resting state after the different training schemes, the training effects and its mechanism of different training schemes;To seek more effective specific training methods as some scientific reasons and references.Objects and Methods:86SPF male SD rats were randomly divided into control, endurance, intermittent, endurance+intermittent, intermittent+endurance groups, After 4weeks, 6weeks, 8weeks to extract the left rectus muscle mitochondria, and to detection the activity of the mitochondrial respiratory chain complexesⅠ, Ⅳ.Results:After 4and 6weeks training, only the activity of complex IV in E6 was significantly higher than S6;After 8weeks training, the complex I of I8 and IE8were significantly higher than S8;the Complex IV of EI8 and I8were significantly higher than that of S8, IE8 was very significantly higher than that of the S8.Conclusion:All of the four training schemes could improve the activity of mitochondrial respiratory chain complexes I and IV, endurance training could improve fastest;Intermittent training could raise anaerobic metabolism more significantly, but the mitochondrial respiratory chain adapted more slowly;IE training might be the most beneficial to improve the aerobic metabolism.

       

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