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    王今越, 王小虹, 冯维斗. 2014: 运动训练抑制了TGFβ通路并缓解了D-半乳糖诱导衰老大鼠的肌肉流失. 体育科学, 34(10): 72-77. DOI: 10.16469/j.css.2014.10.010
    引用本文: 王今越, 王小虹, 冯维斗. 2014: 运动训练抑制了TGFβ通路并缓解了D-半乳糖诱导衰老大鼠的肌肉流失. 体育科学, 34(10): 72-77. DOI: 10.16469/j.css.2014.10.010
    shu
    WANG Jin-yue, WANG Xiao-hong, FENG Wei-dou. 2014: Exercise Trainning Inhibiting TGFβ Pathway and Improving D-Glactose Injection-induced Senescent Muscle Loss in Rat. China Sport Science, 34(10): 72-77. DOI: 10.16469/j.css.2014.10.010
    Citation: WANG Jin-yue, WANG Xiao-hong, FENG Wei-dou. 2014: Exercise Trainning Inhibiting TGFβ Pathway and Improving D-Glactose Injection-induced Senescent Muscle Loss in Rat. China Sport Science, 34(10): 72-77. DOI: 10.16469/j.css.2014.10.010
    shu

    运动训练抑制了TGFβ通路并缓解了D-半乳糖诱导衰老大鼠的肌肉流失

    Exercise Trainning Inhibiting TGFβ Pathway and Improving D-Glactose Injection-induced Senescent Muscle Loss in Rat

      摘要
    • 摘要: 目的:研究TGFβ (经典和非经典) 通路在运动缓解衰老性肌肉流失 (少肌症) 中的作用。方法:24只雄性Wistar大鼠分为3组, C组 (青年安静组) 、S组 (40dD-半乳糖注射致衰老组) 、E组 (40dD-半乳糖注射+6wk跑台运动的衰老运动组) 。检测各组大鼠体重、腓肠肌重量、TGFβ (经典与非经典) 通路因子——TGFβ1、MSTN、Phospho-smad2/3、PhosphoMAPKs (p38、JNK1/2、ERK1/2) 及通路效应因子——p21、Pax7 (WB法) 、MyoD mRNA、MyoG mRNA (RT-PCR法) 。结果:与C组相比, S组腓肠肌比重 (腓肠肌重量/体重) 降低, TGFβ1、MSTN、Phospho-smad2/3、Phospho-MAPKs (Phospho-p38、Phospho-JNK1/2、PhosphoERK1/2) 、p21、MyoD mRNA、MyoG mRNA升高, Pax7降低;与S组相比, E组腓肠肌比重升高, TGFβ1、MSTN、Phospho-smad2/3、Phospho-MAPKs (Phospho-JNK1/2、Phospho-ERK1/2) 降低, p21、Pax7、MyoD mRNA、MyoG mRNA升高。结论:运动训练抑制了TGFβ经典及非经典通路并缓解了衰老肌肉的流失, Pax7、p21、MyoD、MyoG可能作为TGFβ通路的效应器介导了该过程。

       

      Abstract: Objectvie:To explore the functions of (canonical and uncanonical) TGFβpathway in exercise-induced improvement of sarcopenia.Method:24 male wistar rats were divided into 3groups:C group (quiet young group) , S group (40day D-Glactose injection-induced senecence) , E group (40days D-Glactose injection-induced senecence+6weeks exercise trainning on treadmill) .To detect body wet weight, gastrocnemius wet weight (by weighment) , TGFβpathway component-TGFβ1, MSTN, Phospho-smad2/3, Phospho-MAPK (p38, JNK1/2, ERK1/2) , TGFβpathway effector-p21, Pax7 by WB and MyoD mRNA, MyoG mRNA by RT-PCR in each group.Result:The ratio of gastrocnemius weight to body weight decreased, but TGFβ1, MSTN, Phospho-smad2, 3, Phospho-p38, Phospho-JNK1, 2, Phospho-ERK1, 2, p21, MyoD mRNA, MyoG mRNA increased and Pax7 decreased in S group vs C group.The ratio of gastrocnemius weight to body weight increased, but TGFβ1, MSTN, Phospho-smad2, 3, Phospho-JNK1, 2, Phospho-ERK1, 2decreased, Phospho-p38 unchanged, and p21, Pax7, MyoD mRNA, MyoG mRNA increased in E group vs S group.Conclusion:Exericse trainning can improve senescene-induced muscle loss by inhibiting TGFβcanonical and uncanonical pathway with the mediation of Pax7, p21, MyoD and MyoG.

       

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