Abstract: Objective:To observe the effect of SIRT1 of aerobic exercise on the improvement liver lipid metabolism in APOE-/-rats.Methods:16 male C57BL/6Jrats were randomly divided into control group(CC,n=8),exercise group(CE,n=8),16 male APOE-/-rats were randomly divided into control group(AC,n=8),exercise group(AE,n=8).After 12 weeks intervention,hepatic lipid accumulation were observed,Also the protein expression of SIRT1,LKB1,P-LKB1-ser428,AMPKα1 ,P-AMPKα1 -Thr172 and FAS were determined in the liver.Results:1)Compared with CC group,hepatic lipid accumulation increased significantly in the AC group,Compared with AC,lipid accumulation decreased significantly in the AE group.2)Compared with CC group,the content of SIRT1(P<0.05),P-LKB1-ser428(P<0.01)and P-AMPKα1 -Thr172(P <0.01)increased significantly,while the expression of LKB1,AMPKα1 and FAS(P>0.05)didn't change significantly in the CE group.3)Compared with CC group,the expression of SIRT1(P <0.05),P-LKB1-ser-428(P <0.01)and PAMPKα1 -Thr172(P < 0.01)decreased significantly,while the expression of LKB1,AMPKα1 and FAS(P>0.05)didn't change significantly in the liver of AC group.4)Compared with AC group,the content of SIRT1(P<0.01),P-LKB1-ser428(P<0.01)and PAMPKα1 -Thr172(P<0.01)increased significantly,while that of FAS(P<0.01)decreased significantly,and that of LKB1 and AMPKα1 didn't change obviously(P>0.05).Conclusions:12 weeks aerobic exercise may be through promoting the SIRT1,increasing LKB1 and AMPKα1 activity,inhibiting the expression of FAS,to ameliorate hepatic lipid metabolism disorder of APOE-/-rats.