耐力训练C57BL/6J小鼠工作肌ATGL的变化及信号分子cAMP和PPAR-γ的影响
The Effect of Signal Molecule cAMP and PPAR-γ on ATGL of Working Muscular in C57BL/6J Mouse in Exercise Training
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摘要: 目的:1) 了解耐力训练不同时间点C57BL/6J小鼠ATGL基因和蛋白表达的变化;2) 腹腔注射c AMP的激活剂Forskolin、PPAR-γ的抑制剂T0070907、观察耐力训练后ATGL的表达, 探究ATGL变化可能的信号分子机制。方法:实验1, C57BL/6J小鼠跑台耐力训练训练4周, 使用实时荧光定量PCR和蛋白质印迹法测定腓肠肌和比目鱼肌ATGL基因和蛋白表达的变化;实验2, 采用跑台耐力训练小鼠4周, 分别每天腹腔注射forskolin、T0070907和DMSO, 测定腓肠肌和比目鱼肌ATGL基因和蛋白表达的变化。结果:1) 耐力运动引起比目鱼肌ATGL基因和蛋白表达量出现上升 (P<0.05) , 并且在第4周运动组的ATGL基因表达量显著高于对照组 (P<0.05) ;第3、第4周ATGL蛋白表达都显著高于对照组 (P<0.05) ;2) 耐力运动时腓肠肌ATGL基因和蛋白表达出现上升 (P<0.05) , 耐力训练第3周和4周ATGL基因和蛋白表达量运动组显著高于对照组 (P<0.05) ;3) 4周的耐力运动训练后, forskolin组的ATGL基因和蛋白表达无论是比目鱼肌还是腓肠肌都出现显著高于对照组 (P<0.05) , T0070907组的腓肠肌中ATGL基因和蛋白表达显著低于对照组 (P<0.05) ;4) 在4周的耐力运动训练后, forskolin组的ATGL基因表达腓肠肌显著高于比目鱼肌 (P<0.05) 。结论:耐力训练会引起C57BL/6J小鼠比目鱼肌和腓肠肌中的ATGL基因和蛋白表达上升, 信号分子c AMP和PPAR-γ参与了这一过程。Abstract: Objective:1) The purpose of this study was to observe the changes of ATGL gene and protein expression in C57BL/6J mice at different time points in endurance training;2) To explore the possible molecular mechanism of ATGL changes after intraperitoneal injection of c AMP activator Forskolin, PPAR-inhibitor T0070907.Methods:Experiment 1, Endurance training last for four weeks of training C57BL/6J mice, the gastrocnemius and soleus muscle of ATGL gene and protein expression was determined by real-time fluorescence quantitative PCR and Western blotting;Experiment 2, ATGL gene and protein expression in gastrocnemius and soleus muscle are determined respectively daily intraperitoneal injection of forskolin, T0070907 and DMSO after 4 weeks treadmill endurance trained mice.Results:1) ATGL gene and protein of the soleus muscle expression increased in endurance exercise (P<0.05) , and the ATGL m RNA expression in exercise group was significantly higher than the control group in fourth week (P<0.05) , the expression of ATGL protein was significantly higher than that of control group in the third, and in the fourth weeks (P<0.05) ;2) ATGL m RNA and protein expression in the gastrocnemius muscle increased during endurance exercise (P<0.05) , ATGL m RNA and protein expression in exercise group was significantly higher than those in the control group in the third week and the fourth week of endurance training (P<0.05) ;3) ATGL gene and protein expression of soleus and gastrocnemius muscle in forskolin group for 4 weeks endurance training were significantly higher than those of the control group (P<0.05) , ATGLm RNA and protein of T0070907 group in the gastrocnemius muscle was significantly lower than those of the control group (P<0.05) ;ATGL gene expression in forskolin group was significantly higher than that of gastrocnemius muscle after 4 weeks endurance training (P<0.05) .Conclusion:Endurance training can lead to the increasement of ATGL gene and protein expression soleus and gastrocnemius muscle in the C57BL/6J mouse, which the c AMP and PPAR-γ, signal molecule are involved in.
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