Abstract: The outstanding performance Chinese Delegation achieved in the Tokyo Olympic Games showed unique advantages of concentrating nationwide effort and resources on developing competitive sports, and also showed achievements of strategic transition from systematic preparation to science and technology preparation for the Olympic Games. The study comprehensively reviewed the main experiences of how China used science and technology to prepare for the Tokyo Olympic Games, and explored the value connotation of it, and look ahead at the trend, orientation and measures of preparation for the Olympic Games with science and technology. With the support of national system and the guidance of President Xi Jinping's “global vision, international standards, Chinese characteristics, high positioning”, Chinese delegation bravely renewed the concept and pattern of preparation, and finished the transition of the concept, mindset, implementation path and training methods. As the major measures of preparation, the potential and vitality of science and technology preparation were inspired by policy and mechanism innovation. The problem solving ability has been advanced through building the multi-functional team, developing hi-tech intellectual equipment, strengthening the combination of general strength and conditioning training and real competition, improving the overall competitive force, and establishing the serving system for both individuals and teams. The system of science and technology preparation for Olympic Games has enlightened us that: We should dynamically realize the new pattern of global competition; we should grasp the elements,structure and standard process of the Olympic preparation by using the methods of systematic science and concept of engineering;we should use the innovative and problem-oriented method to tackle the key problems; we should introduce intellectual equipment actively; we should enhance both general and specific strength and conditioning training, and strengthen competitive ability through combining training and competition.