Abstract: Objective: The movement behavior includes six parts, i. e., daytime sleep duration(DSP), night sleep duration(NSP),sedentary behavior(SB), light physical activity(LPA), medium physical activity(MPA) and vigorous physical activity(VPA), the recommended amounts of these six parts were established and compared with “Movement Behavior Guideline for Chinese Preschoolers in 3~6 Years”. Methods: 530 preschool children were recruited in this study, the accelerometer was used to continuously measure movement behavior status for 7 days, 24 hours per day, and the National Fitness Measurement Standard Manual(preschoolers part) was used to assess their physical fitness level. The partial correlation analysis, ROC analysis and binary logistic regression analysis were used to conduct statistical analysis. Results: 1) After controlling for gender and age, the DSP, LPA,MPA, VPA, MVPA and TPA were positively associated with physical fitness level, while the NSP and SB were negatively associated with physical fitness level; 2) after adjusting gender and age, the MPA, VPA, MVPA and TPA were increased, and the ratio of not reaching physical fitness standard was decreased(OR<1, P<0.05); on the contrary, with the increase of SB level, the ratio of not reaching physical fitness standard was increased(OR>1, P<0.05); 3) all of the SB, LPA, MPA, VPA, MVPA, and TPA have significant distinguishing effect on physical fitness level, the best cut-off value of SB, LPA, MPA, VPA, MVPA and TPA were512 min/d, 176 min/d, 37 min/d, 9 min/d, 46 min/d and 233 min/d, respectively; 4) compared with those who did not meet the recommended physical activity amount, when the recommended amounts of SB, LPA, MPA, VPA, MVPA and TPA in this study were reached, the ratio of not reaching physical fitness standard was decreased(OR<1, P<0.05). Conclusions: compared with“Movement Behavior Guideline for Chinese Preschoolers in 3~6 Years”, the recommended amount of MVPA is lower and the TPA is higher. At the same time, the recommended amounts of LPA, MPA, VPA and SB have been added. However, further research should treat this conclusion carefully, and more indicators and larger samples should be considered to promote the development of related guidelines.