Changes of Serum Hepcidin and the Expression of HJV mRNA in Liver during the Exercise-induced Hypochrosis in Rats

Objective:To discuss the mechanism of iron metabolism during the formation of exercise-induced hypochrosis.Method:36 male Wistar rats were randomly divided into two groups:control group(CG) and exercise-induced hypochrosis group(EG),and were sampled after 3,4 and 5-week running.The expression of hepatic hepcidin mRNA was examined by Real-Time PCR.The status of serum hepcidin was examined using Enzyme linked immunosor-bent assay(ELISA).Results:1) The level of hemoglobin(Hb),red blood cells(RBC),hematocrit(Hct) in EG was significantly decreased compared with CG.2) The content of WBC was significantly increased in 4-week EG and 5-week EG compared with 4-week CG and 5-week CG(P<0.05 and P<0.01).3) The HJV mRNA in liver and serum hepcidin of 3-week EG were significantly lower than that of the 3-week CG(P<0.05).There was no significant changes of HJV mRNA and serum hepcidin between 4-week EG's and CG(P>0.05).The HJV mRNA and serum hepcidin in 5-week EG were significantly higher than those in 5-week CG(P<0.01).Conclusion:The log-term intensity exercise could increase inflammation and expression of hepatic HJV and in turn the expression of hepatic hepcidin mRNA was increased.It may be one of the most important reasons for the formation of the exercise-induced hypochrosis.