Effects of AngiotensinⅡ on Restructuring of Capillarization of the Skeletal Muscle of Amyotrophic Rat

Objective:To study the effect of angiotensinⅡ on the restructuring of capillarization of the skeletal muscle in the process of amyotrophy.Method:female SD rats were randomly divided into four groups:control group (C) , control group with slow-released angiotensinⅡ agent (CA) , tail-suspended group (T) , tail-suspended with slow-released angiotensinⅡagent group (TA) , 8 rats for each group.Myosin-ATPase histochemistry was used to assay the changes of the capillarization of the skeletal muscle, capillary/fiber (C/F) to observe the change of capillary density;Vascular endothelial growth factor (VEGF) and Vascular endothelial growth factor receptor-2 (KDR) protein content were performed by Western blotting and immunohistochemistry.Results:The results of Myosin-ATPase histochemistry:the capillary density (C/F) of CA group was increased significantly than other three groups (P<0.05) , but the C/F of T group was decreased significantly than C group (P<0.05) , and TA group was increased significantly than T group, but there was no significant difference between TA and C group.The results of Western blotting:protein content of VEGF of CA group was decreased significantly than C group, but the protein content of KDR was increased.The content of both VEGF and KDR of TA group was increased significantly than T group (P<0.05) .Conclusion:In normal condition, angiotensinⅡ boost the increase of capillarization of the skeletal muscle in the process of amyotrophy, the capillary density was decreased, angiotensinⅡcan maintain the capillary density of the skeletal muscle in a certain degree.