TLR4 Signaling Pathway Involved in Repairing of Vascular Inflammation Damaged by Aerobic Exercise on ApoE Knock Out in Mice

Objective: Indicated of the effects and mechanisms of atherosclerosis vascular inflammation damaged by medium-intensity aerobic exercise and simvastatin pharmaceutical on ApoE knock out mice.Methods: Induced atherosclerosis model through ApoE gene knockout and high fat and high cholesterol fed in mice,and were divided into atherosclerosis groups,exercise groups,drug groups;ELISA assay viewed of serum inflammations and hormone levels;TLR4,NF-κb,EGF,TGF-βlevels detected by immunohistochemistry assay;RT-PCR assay detection of CRP,MCP-1mRNA expression.Results: The atherosclerosis groups showed obvious plaque tissue endothelial cells on ApoE knock out mice for 12 weeks;the drug groups showed endothelial cell swelling and foamy surface,the exercise groups were no more than organizational changed.The serum of IL-6,TNF-a levels and TLR4,NF-κb,CRP,MCP-1expression in vascular of the exercise and drug groups were lower than the quite groups(P<0.05),while IL-10,ET1,AngⅡ levels and EGF,TGF-β mRNA expression increased(P<0.05).Conclusion: It's effectively improved vascular inflammation damaged by aerobic exercise,and the possible mechanism mediated Toll-like receptor signaling pathway,while restored hormone secretion and promoted vascular endothelial cell regeneration.