Influence of Tea Polyphenols and Endurance Training on the Apoptosis of Liver Cells of Fat-Rich Diet Rats

Objective:To explore the influence of tea polyphenols and endurance training on the apoptosis of liver cells of fat-rich diet rats.Methods:First,64 SD male rats were randomly divided into 2 groups:quietness group and endurance training group.Then each group was divided into 4 subgroups:control group(A and E),fat-rich diet group(B and F),fat-rich-diet and low-tea-polyphenols group(C and G,150mg/kg),fat-rich-diet and high-tea-polyphenols group(D and H,300mg/kg).The training group received a 60min endurance training every day.Six weeks later,the livers of the rats were removed from the body,being observed the histo-structure by the routine stained hematoxylin-eosin(HE),and the variations of apoptois protein expressions by the immunohistochemisty technology,and the differences of the apoptotic index by TUNEL.Results:Observed by HE,obvious steaosis was found in the liver cells of the fat-rich-diet group in the quiet state.Both absorption of tea polyphenols and the endurance training can improve the steatosis,and the high-tea-polyphenols group do better than the low-tea-polyphenols group.If the two combined,the liver cell structure of the fat-rich diet rats can be kept in a normal level.In a quite state,the ratios of Bax/Bcl-2 in the fat-rich diet rats group are higher than those of the control group;the absorption of tea polyphenols results in a down-regulation Bax expression,but exerted no influence on Bcl-2 expression,and both of the ratios going downward and lower than those of the control group.6 weeks endurance training lead to the fall of the Bax and Bcl-2 expression in the liver cells of the fat-rich diet rats,and the ratios of Bax/Bcl-2 has no difference from the control group.Endurance training combined with tea polyphenols absorption causes a downward Bax and Bcl-2 expression,and the ratios of Bax/Bcl-2 dropped apparently,coming back to the same level as that of the quietness group.The fat-rich diet causes a remarkable increase of the apoptosis index of liver cells of the rats,but the endurance training or tea polyphenols can make a decrease of the apoptosis index,and if the two combined together,the apoptosis index can drop further.Conclusion:The fat-rich diet causes steatosis and increase of apoptosis in the liver cells.Both the endurance training and tea polyphenols can decrease the apoptosis of liver cells and improve the cell structure,and if the two combined,a better result is found.