The Effects of Endurance,Strength and Mixing Type Training on Satellite Cell Activation Signaling Pathway in Adult C57BL /6 Mouse Skeletal Muscle

Objective: To investigate the effect of endurance,strength and mixing type training on satellite activation.Methods: 32 three-month-old male C57BL/6 mice were randomly distributed into four groups: sedentary(C,n=8),endurance training(E,n=8),strength training(S,n=8),mixing type training(M,n=8).After 28 weeks exercise intervening,quantitative real-time PCR method is used to detect nNOS,MMP-2,HGF,c-met mRNA expression in tibialis anterior muscles.Results: 1) Compared with the controls,tibialis anterior and the weight ratio increases in three types of exercise training.2) Endurance training significantly increased nNOS,HGF,c-met mRNA transcription except MMP-2 mRNA.3) Strength training significantly increased nNOS,MMP-2,HGF,c-met mRNA transcription.4) Mixing type training significantly increased HGF mRNA transcription,but had no apparent effect on nNOS,MMP-2,HGF,c-met mRNA transcription.Conclusion: Strength training plays an important role in satellite cell activation,which may be important in maintaining the number of satellite cell pool and promoting extensive skeletal muscle hypertrophy.Endurance training can also promote the activation of satellite cells and would also have its merits in the prevention the number of satellite cells reduction and maintenance skeletal muscle mass and strength.The mixed type training cannot play a composite effect of endurance and strength training in the activation process of satellite cell.It suggested that we should consider the intensity,time and frequency when we arrange endurance and strength training to sustain the number of skeletal muscle satellite cells and promote muscle hypertrophy.