Application of Cardiac Molecular Marker NT-proBNP in the Assessment of Cardioprotective Effect of Exercise Preconditioning

Objective: On the basis of the cardioprotective effect of exercise preconditioning(EP),this study was designed to focus cardiac molecular marker NT-proBNP to comprehensively assess the protective effect of EP on the heart undergoing the exhaustive exercise by means of the associated application of NT-proBNP,cTnI and HBFP staining.Methods:One hundred SD rats were randomly assigned to control group,EE group,EP group and EP+EE group.The rat model of EP was set up by means of the single bout of intermittent treadmill exercise characterizing the high intensity,and the rat model of acute myocardial injury in the manner of exhaustive treadmill exercise.The serum concentration of NT-proBNP and cTnI and myocardium staining were measured by ELISA,CLIA or HBFP array,respectively.Results:Compared with Group C,Group EE significantly increased in the serum concentration of NT-proBNP(P<0.05),and Group EP appeared to decrease slightly(P>0.05);Compared with Group EP,Group EP+EE showed a little enhancement(P>0.05);Compared with Group EE,Group EP+EE markedly decreased(P<0.05).The changing tendency of Serum concentration of NT-proBNP and cTnI and myocardium HBFP staining on rats from the different groups was comparably consistent.Conclusions:EP is one of non-injury method of preconditioning,which can significantly attenuate the acute myocardial injury caused by the exhaustive exercise and promote the recovery of the heart function.The associated utilization of Serum concentration of NT-proBNP and cTnI and myocardium staining is well complementary,and is contributed to comprehensively assess the protective effect of EP on the heart undergoing the exhaustive exercise.