The Proteome Variation of Rat Skeletal Muscle Induced by Repeated Eccentric Exercise

Objective:To research proteome variety on skeletal muscle damage repair process induced by one and repeated eccentric exercise and proteomics mechanism of skeletal muscle repairing made by repeated eccentric exercise;Methods:72 Wistar rats were randomly divided into normal control group,one eccentric exercise group and repeat exercise group a week later.Control group was not done exercise,the rest groups were exercised with 18m/min of speed and-16 degrees of slope.Rats moved for 30min,rest for 5min and then moved for 30min.Repetitive motion was done after a week.Proteome extraction and dielectrophoresis were taken after 0h,24h,48h,72h,168h of one and repeated eccentric exercise,specific proteins were identified by MALDI-TOF MS and sorted according to its function.Results:Proteins expression about energy metabolism and cell damage were reduced especially from 0h to 72h after repeated motion.The proteins number of decreased and increased expression were not different obviously from 0h to 24h and the decreased proteins number was more than number of increased proteins obviously at 72h after repeated motion.During process of damage repair,energy metabolism related proteins included isocitrate dehydrogenase,phosphate glucose mutase,nicotinamide adenine dinucleotide dehydrogenase,hemoglobin binding protein,pyruvate dehydrogenase,triose phosphate isomerase;cell damage repair related proteins were consisted of ubiquitin carboxyl terminal hydrolytic enzyme,immune globulin lambda light chain,intracellular chlorine ion channel protein,protein phosphatase 2c protein,glutathione peroxidase,double function peroxidase and express active;skeletal muscle cell structure protein have heat shock protein,endoplasmic reticulum stations protein and keratin,myosin,mitochondrial membrane protein,etc.Conclusion:Repeated eccentric exercise may promote key enzyme expression of energy metabolism and energy supply for damage cell,accelerate skeletal muscle contraction protein degradation and cell swallow,and scavenge free radicals and slow inflammatory reaction during 24⁴8h to speed up the skeletal muscle damage repair.