Effect of Simulated Altitude Training on the Myocardial Mitochondria in Rats and Intervention of Wheat Peptide

Objective:To explore the intervention effect of wheat peptide supplement on myocardial mitochondrial structure and function in simulated altitude training rat and its mechanism.Methods:Used the experimental scheme of interaction design, SD rats were submitted simulated hypoxia (3 000m) and/or exercise training, wheat peptides was supplement after simulated altitude training in every time, after 9 weeks, myocardial mitochondria ultrastructure was observed, and myocardial mitochondria ATPase, SOD activity, MDA and CCO content were measured.Results:1) the long-term exercise training could cause slight damage of myocardial mitochondria, and mitochondrial ATPase activity decreased, MDA content increased significantly, CCO content and SOD activity decreased, but no significant difference.Hypoxic exposure could cause serious injury of myocardial mitochondria, and mitochondrial ATPase, SOD activity and CCO content were significantly decreased, MDA content increased significantly.Hypoxia combined with exercise training could make the myocardial mitochondria injury more serious, and it had significant interaction to further reduce ATPase activity and increase MDA content, but no significant interaction to further reduce CCO content and SOD activity.2) Compared with group HE, myocardial mitochondrial injury in group HEW significantly reduced, and ATPase activity and CCO content were significantly increased, MDA content decreased and SOD activity increased, but there were no significant difference.Conclusion:1) Long-term altitude training can decrease antioxidant capacity of myocardial mitochondria, increase oxygen free radicals, which induce pathological damage of myocardial mitochondria structure and function.But in the two factors of hypoxia and exercise training, the effects of hypoxia on myocardial mitochondrial structure and function occupies the dominant position.2) Wheat peptide supplement can improve myocardial mitochondrial antioxidant ability of simulated altitude training rats, reduce oxygen free radical generation, which has a very important role in reducing myocardial mitochondria injury in altitude training rats, maintaining the normal structure and function of mitochondria.