Study on Growth Factors Protein Expression Characteristics in Tendon of Long Head of Biceps Brachii during Overuse Process

Objective:This experiment was designed to study the role of growth factors in tendon degeneration overuse-induced.Methods:This study established the rats tendon overused model by downhill running, detected growth factors gene expression in various stages of overuse by immunohistochemistry.Results:In whole tendon, bFGF protein expression was significantly increased during the downhill running, but TGFβ1, bFGF and BMP12 had no significantly change.bFGF was significantly increased at 2 weeks (P<0.01) , decreased at 4 weeks, and raised again at 6-8 weeks.In rat biceps brachii tendon intertubercular sulcus parts, IGF1 expression had no significantly change, but expression of TGFβ1, bFGF, and BMP12 were significantly increased.The expression of TGFβ1increased gradually, reached a peak at 6weeks (P<0.05) , decreased rapidly at 8 weeks.The expression of bFGF reached the peak at 4 weeks value (P<0.01) , and then decreased at 8 weeks.The expression of BMP12 protein increased gradually, reached a peak at 6 weeks (P<0.05) , and declined slightly at 8 weeks.Conclusion:During overuse process, long-term abnormal expression of growth factors was an important pathological chang of tendon degeneration, which probably pushed the tendon degeneration process.