Discussion on Effects of Different Aerobic Exercise Intensities on the Hepatitis of NAFLD Rats Based on the SIRT1 Axis

Objective:To study how the aerobic exercise of different intensity has therapy effect on nonalcoholic steatohepatitis of rats model.Methods:Randomly divide the fifty Sprague Dawley rats into Normal Diets group (ND) , High Fat Diet Control group (C) , High Fat Diet Low Intensity Exercise group (LE) , High Fat Diet Middle Intensity Exercise group (ME) and High Fat Diet Incremental intensity Exercise group (IE) , 10 rats in each group.The ND group is feed normal diet and the other groups are high fat diet for 16 weeks.Every exercise groups start to run on the animal treadmill since the 11th week during the experiment for 6weeks.At the end of test, the rat's abdominal aortic blood was to measure the level of ALT, blood fat and adiponectin, morphology of hepatic tissue was observed, the level of SOD, MDA in hepatocytes was assayed and the mRNA of SIRT1, MCP1, NCF2 was measured, and the fat content in liver was observed by PI/OP MRI, as well as the protein expression of SIRT1 and nuclear NF-κB in hepatocyte.Results:The nonalcoholic steatosis hepatitis and hepatic fibrosis was occured in the C group after 16weeks high-fat feeding.However, three types of exercise intensity could ameliorate the lipid deposition and the state of oxidative stress in liver.Compared to the low intensity exercise, middle and incremental intensity exercise could significantly increase the level of serum HWM adiponectin and the protein expression of SIRT1 in hepatocyte, supress the protein expression of NF-κB in nucleus and its downstream such as MCP1 and NCF2 mRNA expression in hepatocyte, so impede the occur of lobuler hepatitis.Conclusion:All three different intensities of aerobic exercise could supress the lipid accumulation in rats liver through weight reduction mechnism.Vigorous aerobic exercise profoundly restrain the transition of NAFLD to NASH of rats through SIRT1 signal transduction axis in hepatocyte, but low intensity of aerobic exercise couldn't.