The Effect of Endurance Exercise on Autophagy and Mitophagy in the Skeletal Muscle of Mice with Alimentary Obesity

CUI Di; QIU Shou-tao; WANG Hai-yan; HE Jie; QI Zheng-tang; SUN Yi; DING Shu-zhe

doi:10.16469/j.css.2014.12.009

China Sport Science > 2014 > 34(12): 63-71. > DOI: 10.16469/j.css.2014.12.009

CUI Di, QIU Shou-tao, WANG Hai-yan, HE Jie, QI Zheng-tang, SUN Yi, DING Shu-zhe. 2014: The Effect of Endurance Exercise on Autophagy and Mitophagy in the Skeletal Muscle of Mice with Alimentary Obesity. China Sport Science, 34(12): 63-71. DOI: 10.16469/j.css.2014.12.009

Citation:

PDF (894 KB)

The Effect of Endurance Exercise on Autophagy and Mitophagy in the Skeletal Muscle of Mice with Alimentary Obesity

Key Laboratory of Adolescent Health Assessment and Exercise Intervention,Ministry of Education,East China Normal University

More Information

Received Date: July 13, 2014

Graphical Abstract

Abstract

Abstract

Objective:To determine the effect of endurance exercise on cellular autophagy and mitophagy in skeletal muscle of obese mice and explore the underlying molecular mechanism.Methods:After the alimentary obesity ICR mice modle constrction, the obese mice were randomly divided into high-fat diet control group (HC, n=6) , normal diet amelioration group (HN, n=6) and normal diet combined with endurance exercise group (HNE, n=6) .The mice of normal diet control group during the constrction of alimentary obesity model were assigned as the normal control group (NC, n=6) .The mice in groups NC, HN and HNE were fed with nomal diet while the mice of group HC were fed with high-fat diet.The mice of group HNE underwent treadmill running for 6weeks, 45min/d, 6times per week.Results:1) High-fat dier for 6week increased the body weight, Lee's index and epididymal fat percentage of mice, while a series of blood index such as BG, TG, TC, HDL-C and LDL-C increased significantly.2) The relative contents of myosin heavy chain Ⅰ, Ⅱa and Ⅱc mRNA were upregulated in group HNE, compared with group HC.3) The mitochondrial membrance potential of skeletal muscle in group HC was lower than that of group NC, while the mtDNA relative content in group HC, HN and HNE were lower than NC.4) Compared to NC, the autophagy related genes ATG13 and LC3 mRNA decreased while P62 mRNA expression level increased;the BECN1, ATG13 and LC3mRNA relative content of HN and HNE were higher than those in HC (P<0.05) and the ratio of LC3Ⅱ/Ⅰof HNE increased in skeletal muscle compared with HC.5) The mitophagy related gene NIX mRNA expression was lower in HC compared to that of NC, whereas it accerlarated in group HN and HNE;in group HNE, the PARK2 and PARL mRNA relative content increased compared to HN;the protein content of PINK1 downregulated in HC and up-regulated obviously in group HN and HNE (P<0.05) .Conclusion:1) Long-term high-fat-diet intervention can lead to alimentary obesity accompanied with metabolic disorders and mitochondrial dysfunction in the skeletal muscle.2) Endurance exercise combined with dietary intervention can effectively slow down the weight gain and increase the protein expression of myosin heavy chain in the alimentarily obese mice.3) Endurance exercise combined with dietary intervention can obviously attenuate the decline of autophagy level induced by high-fat-diet as well as maintain the quality and function of mitochondria in skeletal muscle, in which the mitophagy related PINK1/Parkin singnaling pathway involves and plays a crititial role.
- exercise,
- alimentary obesity,
- autophagy,
- mitophagy,
- PINK1/Parkin

FullText(HTML)

References (49)

References

崔迪, 贺杰, 孙婧瑜, 等.耐力训练与P53抑制剂PFT-α对小鼠骨骼肌线粒体自噬相关基因表达的影响[J].天津体育学院学报, 2013, 28 (5) :422-426.

崔迪, 邱守涛, 漆正堂, 等.Mieap与线粒体质量控制[J].中国运动医学杂志, 2013, 32 (11) :1029-1035.

马国栋, 刘艳环.耐力训练对急性酒精性肝损伤线粒体自噬功能的影响及机制研究[J].体育科学, 2011, 31 (10) :85-90.

漆正堂, 丁树哲.运动适应的细胞信号调控:线粒体的角色转换及其研究展望[J].体育科学, 2013, 33 (7) :65-69.

孙志, 张中成, 刘志诚.营养性肥胖动物模型的实验研究[J].中国药理学通报, 2002, 18 (4) :466-467.

ALERS S, LOFFLER A S, PAASCH F, et al.Atg13and FIP200act independently of Ulk1and Ulk2in autophagy induction[J].Autoph, 2011, 7 (12) :1423-1433.

ASHRAFI G, SCHWARZ T L.The pathways of mitophagy for quality control and clearance of mitochondria[J].Cell Death Differ, 2013, 20 (1) :31-42.

DUVED C, WATTIAUX R.Functions of lysosomes[J].Annu Rev Physiol, 1966, 28:435-492.

DE MOURA M B, DOS SANTOS L S, Van HOUTEN B.Mitochondrial dysfunction in neurodegenerative diseases and cancer[J].Environ Mol Mutagen, 2010, 51 (5) :391-405.

FISCHER F, HAMANN A, OSIEWACZ H D.Mitochondrial quality control:an integrated network of pathways[J].Trends Biochem Sci, 2012, 37 (7) :284-292.

GANLEY I G, LAM DU H, WANG J, et al.ULK1.ATG13.FIP200complex mediates mTOR signaling and is essential for autophagy[J].J Biol Chem, 2009, 284 (18) :12297-12305.

GARBER K.Autophagy.Explaining exercise[J].Sci, 2012, 335 (6066) :281.

GARNOL T, ENDLICHER R, KUCERA O, et al.Impairment of mitochondrial function of rat hepatocytes by high fat diet and oxidative stress[J].Physiol Res, 2014, 63 (2) :271-274.

GOEDECKE J H, MICKLESFIELD L K.The effect of exercise on obesity, body fat distribution and risk for type 2diabetes[J].Med Sport Sci, 2014, 60:82-93.

GOLDSMITH J, LEVINE B, DEBNATH J.Autophagy and cancer metabolism[J].Methods Enzymol, 2014, 542:25-57.

GREEN D R, LEVINE B.To be or not to be?How selective autophagy and cell death govern cell fate[J].Cell, 2014, 157 (1) :65-75.

GREEN D R, Van HOUTEN B.SnapShot:Mitochondrial quality control[J].Cell, 2011, 147 (4) :950-951.

GRUMATI P, COLETTO L, SCHIAVINATO A, et al.Physical exercise stimulates autophagy in normal skeletal muscles but is detrimental for collagen VI-deficient muscles[J].Autophagy, 2011, 7 (12) :1415-1423.

GUO R, ZHANG Y, TURDI S, et al.Adiponectin knockout accentuates high fat diet-induced obesity and cardiac dysfunction:role of autophagy[J].Biochim Biophys Acta, 2013, 1832 (8) :1136-1148.

HE C, BASSIK M C, MORESI V, et al.Exercise-induced BCL2-regulated autophagy is required for muscle glucose homeostasis[J].Nature, 2012, 481 (7382) :511-515.

HE C, SUMPTER R J R, LEVINE B.Exercise induces autophagy in peripheral tissues and in the brain[J].Autoph, 2012, 8 (10) :1548-1551.

HIROTA Y, KANG D, KANKI T.The physiological role of mitophagy:new insights into phosphorylation events[J].Int J Cell Biol, 2012, 2012:354914.

HOSHINO A, MATOBA S, IWAI-KANAI E, et al.P53-TIGAR axis attenuates mitophagy to exacerbate cardiac damage after ischemia[J].J Mol Cell Cardiol, 2012, 52 (1) :175-184.

HU C C, QING K, CHEN Y.Diet-induced changes in stearoylCoA desaturase 1expression in obesity-prone and-resistant mice[J].Obes Res, 2004, 12 (8) :1264-1270.

JAMART C, BENOIT N, RAYMACKERS J M, et al.Autophagy-related and autophagy-regulatory genes are induced in human muscle after ultraendurance exercise[J].Eur J Appl Physiol, 2012, 112 (8) :3173-3177.

KABEYA Y, MIZUSHIMA N, YAMAMOTO A, et al.LC3, GABARAP and GATE16localize to autophagosomal membrane depending on form-II formation[J].J Cell Sci, 2004, 117 (13) :2805-2812.

KIM I, RODRIGUEZ-ENRIQUEZ S, LEMASTERS J J.Selective degradation of mitochondria by mitophagy[J].Arch Biochem Biophys, 2007, 462 (2) :245-253.

KUBLI D A, GUSTAFSSON A B.Mitochondria and mitophagy:the yin and yang of cell death control[J].Circ Res, 2012, 111 (9) :1208-1221.

LEE Y, KIM J H, HONG Y, et al.Prophylactic effects of swimming exercise on autophagy-induced muscle atrophy in diabetic rats[J].Lab Anim Res, 2012, 28 (3) :171-179.

LEVINE B, KROEMER G.Autophagy in the pathogenesis of disease[J].Cell, 2008, 132 (1) :27-42.

LIU X, YE B, MILLER S, et al.Ablation of ALCAT1mitigates hypertrophic cardiomyopathy through effects on oxidative stress and mitophagy[J].Mol Cell Biol, 2012, 32 (21) :4493-4504.

LOPEZ-MIRANDA J, PEREZ-MARTINEZ P, MARIN C, et al.Dietary fat, genes and insulin sensitivity[J].J Mol Med (Berl) , 2007, 85 (3) :213-226.

LOWELL B B, SHULMAN G I.Mitochondrial dysfunction and type 2diabetes[J].Sci, 2005, 307 (5708) :384-387.

NAIR U, KLIONSKY D J.Activation of autophagy is required for muscle homeostasis during physical exercise[J].Autophagy, 2011, 7 (12) :1405-1406.

NAKAMURA Y, KITAMURA N, SHINOGI D, et al.BNIP3and NIX mediate Mieap-induced accumulation of lysosomal proteins within mitochondria[J].PLoS One, 2012, 7 (1) :e30767.

O'LEARY M F, VAINSHTEIN A, IQBAL S, et al.Adaptive plasticity of autophagic proteins to denervation in aging skeletal muscle[J].Am J Physiol Cell Physiol, 2013, 304 (5) :C422-C430.

RASMUSSEN H N, ANDERSEN A J, RASMUSSEN U F.Optimization of preparation of mitochondria from 25-100 mg skeletal muscle[J].Anal Biochem, 1997, 252 (1) :153-159.

RUBINSZTEIN D C, MARINO G, KROEMER G.Autophagy and aging[J].Cell, 2011, 146 (5) :682-695.

RUOHONEN S T, VAHATALO L H, SAVONTAUS E.Dietinduced obesity in mice overexpressing neuropeptide y in noradrenergic neurons[J].Int J Pept, 2012, 2012:452524.

RUSSELL A P, FOLETTA V C, SNOW R J, et al.Skeletal muscle mitochondria:a major player in exercise, health and disease[J].Biochim Biophys Acta, 2014, 1840 (4) :1276-1284.

SINGH K K.Mitochondrial dysfunction is a common phenotype in aging and cancer[J].Ann N Y Acad Sci, 2004, 1019:260-264.

SMUDER A J, KAVAZIS A N, MIN K, et al.Exercise protects against doxorubicin-induced markers of autophagy signaling in skeletal muscle[J].J Appl Physiol (1985) , 2011, 111 (4) :1190-1198.

TANIDA I.Autophagosome formation and molecular mechanism of autophagy[J].Antioxid Redox Signal, 2011, 14 (11) :2201-2214.

TONG J, YAN X, YU L.The late stage of autophagy:cellular events and molecular regulation[J].Protein Cell, 2010, 1 (10) :907-915.

WHITWORTH A J, PALLANCK L J.The PINK1/Parkin pathway:a mitochondrial quality control system?[J].J Bioenerg Biomembr, 2009, 41 (6) :499-503.

WIRAWAN E, VANDEN BERGHE T, LIPPENS S, et al.Autophagy:for better or for worse[J].Cell Res, 2012, 22 (1) :43-61.

WOHLGEMUTH S E, SEO A Y, MARZETTI E, et al.Skeletal muscle autophagy and apoptosis during aging:effects of calorie restriction and life-long exercise[J].Exp Gerontol, 2010, 45 (2) :138-148.

YOULE R J, NARENDRA D P.Mechanisms of mitophagy[J].Nat Rev Mol Cell Biol, 2011, 12 (1) :9-14.

ZHANG J, NEY P A.Role of BNIP3and NIX in cell death, autophagy, and mitophagy[J].Cell Death Differ, 2009, 16 (7) :939-946.

Supplements (0)

Cited By

Get Citation

PDF

XML

Article views (7) PDF downloads (0)

Turn off MathJax

Article Contents

Abstract

References

	JIANG Ya-bin, ZHANG En-li, REN Bo, WANG Meng. 2022: The Legal Dilemmas and Response Options of Sports Industry’s Digitalization——In the Perspective of Data Factors. China Sport Science, 42(6): 3-10,41. DOI: 10.16469/j.css.202206001
	LIU Bo-han. 2020: Some Legal Issues in Establishing China Sports Industry Investment Fund. China Sport Science, 40(3): 90-97. DOI: 10.16469/j.css.202003009
	WANG Jia-hong, LIU Guang-fei, ZHAO Yi, CHEN Hua-rong, GU De-hong, JIANG Xi, HUANG Shi-chang, CAI Peng-long, QIU Lin, XIONG Ying-zi, SHAO Wei-yu, LIANG Wei, SONG Bin-ling, GAO Yue, WANG Qian. 2019: Research on Legal Issues Related to the Deepening Reform of China’s Sports. China Sport Science, 39(11): 3-14. DOI: 10.16469/j.css.201911001
	YU Shan-xu. 2018: Thoughts on the Process of Chinese Sports Legalization. China Sport Science, 38(7): 20-21. DOI: 10.16469/j.css.201807012
	CHEN Hua-rong. 2017: Research on the Policy and Legal System of Sports For All National Strategy. China Sport Science, 37(4): 74-86. DOI: 10.16469/j.css.201704009
	YAO He-hui. 2015: On the Defects and Improvement of the Legal Protection System about the Kinds of Sports Programs. China Sport Science, 35(5): 10-15,97. DOI: 10.16469/j.css.201505002
	ZHU Wen-ying. 2014: The Legal Application on the Professional Football Player Transfers. China Sport Science, 34(1): 41-47. DOI: 10.16469/j.css.2014.01.003
	ZHANG Wen-wen, WU Yi-hua. 2014: The Basic Legal Issues of International Sports Organization from the View of Legal Pluralism. China Sport Science, 34(1): 14-20. DOI: 10.16469/j.css.2014.01.007
	YUAN Shao-yi. 2013: Discussion on Classification of Civil and Commercial Legal Relations of Sports Events. China Sport Science, 33(3): 3-12. DOI: 10.16469/j.css.2013.03.001
	MA Hong-jun. 2013: Research on Legal Regulation of the Government Sports Public Service System. China Sport Science, 33(1): 3-9. DOI: 10.16469/j.css.2013.01.005

The Effect of Endurance Exercise on Autophagy and Mitophagy in the Skeletal Muscle of Mice with Alimentary Obesity

Abstract

References

Related Articles

Catalog

The Effect of Endurance Exercise on Autophagy and Mitophagy in the Skeletal Muscle of Mice with Alimentary Obesity

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content