Detection of Human Homologous Blood Transfusion by Flow Cytometer

Objective:Blood transfusion could increase red blood cells(RBCs)rapidly and may enhance endurance performance significantly.A validation was carried out to prove the reliability and operability of the detection of homologous blood transfusion with flow cytometry technology.Method:Homologous blood transfusion was detected using flow cytometry technique to observe the presence of different RBCs phenotypes of eight different antigens in a blood sample.The analysis of 46 blood samples containing different percentages(0~5%)of homologous RBCs was carried out by three independent analysts.Results:The method afforded satisfactory results in terms of robustness,sensitivity,specificity,precision and stability.No false positive results were observed.All samples contained 3% homologous RBCs were unambiguously detected.These samples were stable over 4 weeks after using cell stable buffer at 4~8℃.Conclusion:This method fulfils the ISO-17025 accreditation and has been accredited by China National Accreditation Service for Conformity Assessment(CNAS).The method has been successfully utilized to the detection of homologous blood transfusion in major events and routine doping-control samples.