Effects of Combination of Treadmill Exercise and Massage on Inflammation Development and Satellite Cell Proliferation in Rehabilitation Process of Rats Acute Skeletal Muscle Injury

Objective:To investigate the effect of the combination of treadmill exercise and massage on the inflammation NADH of the damage tissue and the expression of nNOS、HGF mRNA in the satellite cell proliferation on the rehabilitation process of rats acute skeletal muscle injury so that to explore the role of massage on the rehabilitation to acute skeletal muscle.Methods:76 adult male SD rats were randomly divided into 5groups:normal control(group A,n=4),self-healing group(group B,n=20),control group of massage(group C,n=16),control of treadmill exercise(group D,n=16),control of mixture(group E,n=16).In group A,nothing were done;In group B,C,D and E,rats were made to the model of rats with acute muscle injury by the improved traumatizing unit.Group B were not given the treatment,massage were given to the group C,E and treadmill exercise were given to the group D,E after three days of the modeling.At 7,14,21,28 days after the modeling,animal gastrocnemius samples were token in group B,C,D and E respectively,HE staining was employed to inspect pathological changes.the inflammation NADH was detected by the Western Blot,Real-time PCR was applied to detect the expression of nNOS,HGF mRNA in muscle satellite cells.Results:After the combination interventionof massage and treadmill exercise,the expression of NADH,nNOS,HGF mRNA was higher in group C,D,E than the group B,and it has highly significant differences(P<0.01).Compare to the group C and D,the expression of NADH,nNOS,HGF mRNA was more in the group E,and it has highly significant differences(P<0.01).Compare to the group C,the expression of nNOS,HGF mRNA of in Group D was higher,and it has significant differences(P<0.05).HE staining shows that myofilaments were disorder and ruptured,inflammatory cells were infiltrated,groud A was not.Compare to the group B,group C,D,E has more blood supply,more myofilaments,more activated muscle satellite cells,necrotic tissue was rehabilitated faster compare to group B.Conclusion:The combination of treadmill exercise can effectively improve the expression level of the NADH,nNOS,HGF mRNA in the muscle satellite cells,promote the inflammation to fade,the proliferation of satellite cell,provide the speed of the rehabilitation and regeneration in the damaged skeletal muscle.