Effects of Interval Exercise on the Expression of Myocardial LIF and LIFR and Cell Apoptosis in a Rat Model with Myocardial Infarction

Objectives:This study was carried out to determine the effects of interval exercise on myocardial cell apoptosis,expression of Leukemia inhibitory factor(LIF)and its reporter in rat with myocardial infarction(MI).Methods:Adult male sprague-dawley rats,3-month old,were randomly divided into three groups:Sham-operated group(Sham),sedentary MI group(MI)and MI with interval exercise group(ME).The MI model was established by ligation of the left anterior descending(LAD)coronary artery,and rats in ME were subjected to 8weeks interval training.Cardiac function,heart tissue remodeling and cardiomyocyte apoptosis were evaluated after the training.The expression of LIF,LIFR,p-STAT3 and STAT3 was determined in the peri-infarcted area of the left ventricle by Western Blotting or RT-qPCR.Results:MI resulted in cardiac dysfunction and myocardial fibrosis,increasing the degree of myocardial cell apoptosis.Compared with MI group,interval exercise signaficantly improved cardiac function,attenuated myocardial remodeling by reducing CVF% and inhibited cardiomyocyte apoptosis in MI rats.Meanwhile,interval exercise training up-regulated the expression of LIF protein and lif and lifr mRNA,and activated the phosphorylation of STAT 3in myocardium of MI rats.Conclusion:Interval exercise inhibits myocardial cell apoptosis,up-regulates the expression of myocardial LIFs,and activates the signaling pathway of LIF/LIFR/STAT 3after MI.