Aerobic Interval Training Activates the Renal miR-21/SIRT1/NF-κB Signalling Pathway and Improves Renal Function in a Rat Model with Myocardial Infarction

Objective:To determine the effects of aerobic interval training (AIT) on the expressions of renal miR-21, SIRT1, Ac-NF-κB and AQP2 in rats with myocardial infarction (MI) . Methods:male Sprague Dawley rats were randomly divided into Sham-operated group (Sham) , Sedentary MI group (MI) and MI with AIT group (ME) (n=12) . The MI model was established by ligation the left anterior descending coronary artery (LAD) , and rats in ME were subjected to 8 weeks treadmill exercise training. After training, cardiaorenal function was evaluated. The localization of AQP2 expression was determined by immunofluorescence staining. The levels of serum AVP and urine AQP2 were assessed by ELISA. The expression of renal AQP2, V2 R and miR-21 was examined by RT-q PCR. The expression of renal SIRT1, Ac-NF-κBp65 and NF-κBp65 protein was examined by western blotting. Results:MI increased the expression of renal AVP, V2 R and AQP2 and the level of urine AQP2, and then increased water reabsorption, thereby resulted in renal dysfunction. Compared with MI group, AIT obviously improved renal function and ameliorated the expression of renal AVP, V2 R and AQP2 and the level of urine AQP2. Meanwhile, AIT up-regulated the expression of renal miR-21 and SIRT1, and inhibited the acetylation of NF-κBp65 in kidney of rats with MI. miR-21 expression was positively related to the expression of SIRT1. Conclusion:AIT up-regulates the expression of renal miR-21, activates miR-21/SIRT1/NF-κB signalling pathway, and then improve the renal function in rats after MI. The protective effect of AIT on renal function in MI rats was related to the increased expression of miR-21 and the activation of the miR-21/SIRT1/NF-κB signaling pathway.