Regulation of Moderate Intensity Swimming Exercise on Hippocampal Synaptic Plasticity
-
Graphical Abstract
-
Abstract
Objective: To explore the timeliness and continuity characteristics about the influence of moderate intensity swimming on hippocampal synaptic plasticity. Methods: 48 male SD rats were randomly divided into control group (CG) , 3 days ( 3 D) , 7 days ( 7 D) , 15 days (15 D) , 5 weeks ( 5 W) exercise groups and 1 week delayed group after 5 weeks exercise ( P5W) . Immunofluorescence, electron microscopy and Western blot technology were used to detect the cell proliferation, synaptic plasticity and synaptic structure associated protein expression respectively in the hippocampal tissue of different swimming rat groups. Results: The proliferation rate (%) of hippocampal dentate gyrus granular cell were 31.70± 1.57, 40.19± 2.40, 36.74± 4.06, 51.70±5.93, 52.90± 6.10, 51.97± 1.47 of CG, 3 D, 7 D, 15 D, 5 W, P5W group respectively, the proliferation rate of 15 D, 5 W, P5W groups were significantly higher than that of control group (P<0.05) . The synapse numbers and PSD thickness of 5 W, P5W groups were significantly higher than that in CG group (P<0.05 or P<0.001) , the synaptic cleft width of 5 W, P5W groups was significantly lower than that in CG group (P<0.05 or P<0.001) , the PSD thickness of 15 D group was significantly higher than that of CG group (P<0.05) . The expression of hippocampal structural protein MAP2 in 5 W, P5W groups was significantly higher than that in CG group (P<0.05 or P<0.001) , and the expression of SYP in 15 D, 5 W, P5W groups was significantly higher than that in CG group (P<0.05) . Conclusions: The hippocampal plasticity induced by moderate intensity swimming has time dependent characteristic and the synaptic plasticity induced by exercise has continuity after the cessation of exercise.
-
-