Effects of 8-Weeks’ Aerobic Exercise on Vascular Endothelial Inflammation and microRNA-126 in Obese Mice

Objective: To investigate the effect of aerobic exercise on vascular endothelial molecular adhesion and inflammation in obese mice. Methods: Thirty-seven SPF male C57BL/6 mice were randomly divided into control diet (CD, n=17) and high-fat diet (HFD, n=20) , and separately fed ordinary food and high fat diet. After 12 weeks, 17 obese mice were randomly divided into obesity control group (OC, n=8) and obese exercise group (OE, n=9) . Seventeen control mice were randomly selected. Randomly divided into normal control group (NC, n = 8) , normal exercise group (NE, n = 9) . The OE group and the NE group performed aerobic exercise for 8 weeks. The treadmill exercise lasted 8 weeks, 6 times a week, 30 minutes each time, and the running speed was 20 m/min. The body weight and abdominal fat content of mice were measured; Morphological changes of mouse thoracic aorta endothelial cells were observed by transmission electron microscopy; Serum TNF-α and insulin levels were measured by ELISA; Real-time PCR and immunohistochemistry were used to measure microRNA-126 (miR-126) , TNF-α, NF-κB, VCAM-1 m RNA. Results: Compared with NC group, mice in OC group's body weight and peritoneal fat were increased (P<0.01) , serum TNF-α level was significantly increased (P<0.01) , transmission electron microscopy showed that the morphology of endothelial cells in obese mice corresponding injury, miR-126 levels in thoracic aorta decreased (P<0.01) , TNF-α, NF-κB, and VCAM-1 protein expression increased (P<0.01) . Compared with OC group, mice in OE group's body weight and lee's index in the decreased (P<0.01) , serum TNF-α levels were down-regulated (P<0.01) , inflammation levels decreased, and endothelial cell morphology was impaired in the OE group. Mi R in the OE group Level of-126 increased (P<0.01) , TNF-α (P<0.01) , NF-κB (P<0.01) , and VCAM-1 (P<0.05) protein expression were down-regulated after exercise. There was a significant correlation with the expression changes of TNF-α (r=-0.60, P<0.05) and VCAM-1 (r=0.69, P<0.05) . Conclusion: 8-weeks' exercise significantly reduced serum inflammatory factors, released endothelial cell molecular adhesion and improved vascular endothelial function in obese mice, which may be related to exercise-induced miR-126 expression.