Effect and Mechanism of Blood Flow Restriction Combined with Low Load Training on Blood Pressure of Male Spontaneously Hypertensive Rats

TAN Chao-wen; ZHAO Yan; YU Ying-ying; MA Xiao-huan; ZHENG Yu-chan

doi:10.16469/j.css.202003005

China Sport Science > 2020 > 40(3): 46-53,63. > DOI: 10.16469/j.css.202003005

TAN Chao-wen, ZHAO Yan, YU Ying-ying, MA Xiao-huan, ZHENG Yu-chan. 2020: Effect and Mechanism of Blood Flow Restriction Combined with Low Load Training on Blood Pressure of Male Spontaneously Hypertensive Rats. China Sport Science, 40(3): 46-53,63. DOI: 10.16469/j.css.202003005

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Effect and Mechanism of Blood Flow Restriction Combined with Low Load Training on Blood Pressure of Male Spontaneously Hypertensive Rats

1. Graduate Department of Nanjing Sport Institute;
2. Nanjing Sport Institute

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Received Date: September 26, 2019

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Abstract

Abstract

To explore the effect and mechanism of blood flow restriction combined with low intensity resistance exercise training on blood pressure in spontaneously hypertensive rats. Four weeks old male spontaneously hypertensive rats were randomly divided into control group, low intensity training group, pressure training group and high intensity training group. 35% ~ 55% 1 RM progressive anti ladder training program was conducted in the low intensity training group; 30% ~ 40% blood flow restriction and 35% ~ 55%1 RM progressive anti ladder training program was conducted in the pressure training group; 55% ~ 75% 1 RM progressive anti ladder training program was conducted in the high intensity training group. After training, the blood pressure, expression of endothelin-1,vascular endothelial growth factor and nitric oxide synthetase were measured. Results:1) compared with the control group and the high-intensity training group, the systolic and diastolic blood pressure were decreased significantly in the pressure training group(P < 0.05); compared with the low-intensity training group, the diastolic blood pressure was decreased significantly in the pressure training group(P < 0.05). 2) compared with the control group, the expression levels of endothelin-1 in the blood was decreased significantly in the pressure training group(P < 0.05), and the expression level of vascular endothelial growth factor and nitric oxide synthetase were increased significantly(P < 0.05); 3) compared with the control group, the expression level of endothelial nitric oxide synthetase in the myocardium was increased significantly in the pressure training group(P < 0.05); 4) in the pressure training group, the systolic pressure was positively correlated with endothelin-1(P < 0.05); the systolic blood pressure was negatively correlated with nitric oxide synthetase, vascular endothelial growth factor and endothelial nitric oxide synthetase(P <0.05). Conclusinon:1) the effect of blood flow restriction combined with low intensity resistance training on blood pressure is better than that of high-intensity resistance training; 2) blood flow restriction combined with low intensity resistance training can increase the expression of vascular endothelial growth factor and nitric oxide synthetase in blood and increase the expression of endothelial nitric oxide synthetase in heart muscle, which result in the improvement of the endothelial cells function and blood pressure.

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References

叶芳,柏平,曾凡星,等,2016.不同强度运动对自发性高血压大鼠血管内皮结构及功能影响的氧化应激机制[J].体育科学,36(12):58-66.

ARAUJO J P,SILVA E D,SILVA J C,et al.,2014.The acute effect of resistance exercise with blood flow restriction with hemodynamic variables on hypertensive subjects[J].J Hum Kinet,12;43:79-85.

BATTAULT S,SINGH F,GAYRARD S,2016.Endothelial function does not improve with high-intensity continuous exercise training in SHR:Implications of eNOS uncoupling[J].Hypertens Res,39(2):70-78.

BERENYIOVA A,et al.,2018.The effect of chronic NO synthase inhibition on the vasoactive and structural properties of thoracic aorta,NO synthase activity,and oxidative stress biomarkers in Young SHR[J].Oxid Med Cell Longevity,1-10.

BOENO F P,et al.,2018.Efeito agudo do exercício de força com restrição do fluxo sanguíneo sobre parâmetros antioxidantes em indivíduos jovens saudáveis[J].J Vasc Bras,17(2):122-127.

BOUTCHER Y N,BOUTCHER S H,2017.Exercise intensity and hypertension:What’s new[J].J Hum Hypertens,31(3):157-164.

CHANG C,et al.,2017.3-Methylcholanthrene/Aryl-hydrocarbon receptor-mediated hypertension through eNOS inactivation[J].JCell Physiol,232(5):1020-1029.

CHAO J,et al.,2017.Opposing effects of oxygen regulation on kallistatin expression:Kallistatin as a novel mediator of oxygeninduced HIF-1-eNOS-NO pathway[J].Oxid Med Cell Longev,1-8.

CHIGNALIA A Z,et al.,2018.Pressure-dependent NOS activation contributes to endothelial hyperpermeability in a model of acute heart failure[J].Bioscience Rep,386:BSR20181239.

COLOMBO R,et al.,2016.Exercise training contributes to H2O2/VEGF signaling in the lung of rats with monocrotaline-induced pulmonary hypertension[J].Vasc Pharmacol,87:49-59.

DESCHENES M R,ROBY M A,GLASS E K,2011.Aging influences adaptations of the neuromuscular junction to endurance training[J].Neuroscience,190:56-66.

FANG T,et al.,2019.Atorvastatin prevents myocardial fibrosis in spontaneous hypertension via interleukin-6(IL-6)/Signal transducer and activator of transcription 3(STAT3)/endothelin-1(ET-1)pathway[J].Med Sci Monit,25:318-323.

GU X,et al.,2015.Inverse correlation between plasma adropin and ET-1 levels in essential hypertension[J].Medicine,94(40):e1712.

LADLOW P,et al.,2018.Low-load resistance training with blood flow restriction improves clinical outcomes in musculoskeletal rehabilitation:A single-blind randomized controlled trial[J].Front Physiol,9:1269.

LI M,et al.,2015.Resistance exercise training increase activation of AKT-eNOS and Ref-1 expression by FOXO-1 activation in aorta of F344 rats[J].J Exerc Nutr Biochem,19(3):165-171.

LI N,et al.,2019.Chronic Intermittent hypobaric hypoxia ameliorates renal vascular hypertension through Up-regulating NOSin nucleus tractus solitarii[J].Neurosci Bull,35(1):79-90.

MARUF F A,AKINPELU A O,SALAKO B L,et al.,2016.Effects of aerobic dance training on blood pressure in individuals with uncontrolled hypertension on two antihypertensive drugs:A randomized clinical trial[J].J Am Soc Hypertens,10(4):336-345.

MATHIEN G M,TERJUNG R L,1986.Influence of training following bilateral stenosis of the femoral artery in rats[J].Am JPhysiol,250:H1050-H1059.

MORAES-SILVA I C,MOSTARDA C T,SILVA-FILHO A C,et al.,2017.Hypertension and exercise training:Evidence from clinical studies[J].Adv Exp Med Biol,1000:65-84.

MORENO M U,et al.,2017.The hypertensive myocardium[J].Med Clin North Am,101(1):43-52.

NADERI-BOLDAJI V,et al.,2018.The effect of blood flow restriction along with low-intensity exercise on cardiac structure and function in aging rat:Role of angiogenesis[J].Life Sci,209:202-209.

NETO G R,SOUSA M S,COSTA P B,et al.,2015.Hypotensive effects of resistance exercises with blood flow restriction[J].J Strength Cond Res,29(4):1064-1070.

PICON M M,et al.,2018.Acute Cardiovascular responses after a single bout of blood flow restriction training[J].Int J Exerc Sci,11(2):20.

PINTO R R,KARABULUT M,POTON R,et al.,2018.Acute resistance exercise with blood flow restriction in elderly hypertensive women:Haemodynamic,rating of perceived exertion and blood lactate[J].Clin Physiol Funct Imaging,38(1):17-24.

PUCCI G,et al.,2019.Acute blood pressure elevation associated with biological therapies for cancer:A focus on VEGF signaling pathway inhibitors[J].Expert Opin Biol Th,19(5):433-442.

OZAKI H,et al.,2013.Effects of high-intensity and blood flowrestricted low-intensity resistance training on carotid arterial compliance:Role of blood pressure during training sessions[J].Eur JAppl Physiol,113(1):167-174.

RAJI-AMIRHASANI A,et al.,2018.Mild exercise along with limb blood-flow restriction modulates the electrocardiogram,angiotensin,and apelin receptors of the heart in aging rats[J].Iran J Basic Med Sci,21(6):558-563.

SANCHES,IRIS,CONTI,et al.,2013.Standardization of resistance exercise training:Effects in diabetic ovariectomized rats[J].Int JSports Med,doi: 10.1055/s-0033-1351254.

SATO Y,2005.The history and future of KAATSU Training[J].Int JKAATSU Training Res,1(1):1-5.

SHARMAN J E,LA GERCHE A,COOMBES J S,2015.Exercise and cardiovascular risk in patients with hypertension[J].Am JHypertens,28(2):147-158.

SHIMOJO G L,et al.,2015.Dynamic resistance training decreases sympathetic tone in hypertensive ovariectomized rats[J].Braz JMed Biol Res,48(6):523-527.

SILVA J F,et al.,2016.Obesity,Inflammation,and exercise training:Relative contribution of iNOS and eNOS in the modulation of vascular function in the mouse aorta[J].Front Physiol,7.

SON W,et al.,2017.Combined exercise reduces arterial stiffness,blood pressure,and blood markers for cardiovascular risk in postmenopausal women with hypertension[J].Menopause,24(3):262-268.

SPRANGER M D,KRISHNAN A C,LEVY P D,et al.,2015.Blood flow restriction training and the exercise pressor reflex:A call for concern[J].Am J Physiol Heart Circ Physiol,309(9):H1440-52.

STANISIC J,KORICANAC G,KOSTIC M,et al.,2019.Lowintensity exercise in the prevention of cardiac insulin resistancerelated inflammation and disturbances in NOS and MMP-9regulation in fructose-fed ovariectomized rats[J].Appl Physiol Nutr Metab,44(11):1219-1229.

SUVORSVA T,PICK S,KOJDA G,2017.Selective impairment of blood pressure reduction by endothelial nitric oxide synthase dimer destabilization in mice[J].J Hypertens,35(1):76-88.

TORPEL A,et al.,2018.Strengthening the brain-is resistance training with blood flow restriction an effective strategy for cognitive improvement[J].J Clin Med,7(10):337.

TOUYZ R M,et al.,2017.Recent advances in hypertension and cardiovascular toxicities with vascular endothelial growth factor inhibition[J].Hypertension,70(2):220-226.

ÜLKER S N,G KOCER,ÜKŞENTURK,2017.Carbon monoxide does not contribute to vascular tonus improvement in exercise-trained rats with chronic nitric oxide synthase inhibition[J].Nitric Oxide,65:60-67.

VIBOOLVORAKUL S,S PATUMRAJ,2014.Exercise training could improve age-related changes in cerebral blood flow and capillary vascularity through the Upregulation of VEGF and eNOS[J].Biomed Res Int,1-12.

WANG L,Y WANG,Z LEI,2019.Chrysin ameliorates ANTU-induced pulmonary edema and pulmonary arterial hypertension via modulation of VEGF and eNOs[J].J Biochem Mol Toxicol,e22332.

YANG H,OGILVIE R W,TERJUNG R L,1991.Low-intensity training produces muscle adaptations in rats with femoral artery stenosis[J].J Appl Physiol,71:1822-1829.

YU Y,GAO Q,XIA W,et al.,2018.Association between physical exercise and biomarkers of oxidative stress among middle-aged and elderly community residents with essential hypertension in China[J].Biomed Res Int,3:4135104.

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	YE Fang, BO Ping, CENG Fan-xing, SHI Li-jun. 2016: Oxidative Stress Mechanisms of Treadmill with Different Intensity on Endothelial Function and Ultrastructure in Spontaneously Hypertensive Rats. China Sport Science, 36(12): 58-66. DOI: 10.16469/j.css.201612008
	ZHANG Xian-liang, XU Bo, PENG Hai-xia, HE Qiang, HE Biao, JI Liu. 2015: Effects of Acute Swimming Exercise on Microglia Activation,Inflammation and Oxidative Stress in the Hippocampus of High-Fat Diet-induced Obese Mice. China Sport Science, 35(9): 58-64,96. DOI: 10.16469/j.css.201509007
	CUI Jian-mei, SU Xiao-yun, WANG Xin, HE Ji-ping. 2014: Effects of Voluntary Wheeling on Behavior Changes,Brain Neuropeptide Y and nNOS Expression within Central Amygdala in the Depressive Model Rats. China Sport Science, 34(5): 15-22. DOI: 10.16469/j.css.2014.05.004
	CHEN Ting, HU De-er-chao-lu, ZHANG Ke, TIAN Zhen-jun. 2013: The Effect of Aerobic Exercise on Beta3-Adrenoreceptor and Nitric Oxide Synthase in Left Ventricular after Myocardial Infarction. China Sport Science, 33(9): 57-63,76. DOI: 10.16469/j.css.2013.09.011
	ZHANG Li, LI Yan-chun, YI Long-yan, NIE Jing, WANG Jing-ling, HU Yang. 2012: Research on Genetic Markers for HiHiLo Training Response in EDN1 and NOS2A Genes. China Sport Science, 32(4): 41-52,76. DOI: 10.16469/j.css.2012.04.001
	LUO Li, ZHANG Jian, DONG Yu, SUN Zhong-wen, LI Xiao-ning, LU A-ming, LIU Cheng-yi. 2011: The Expression of IGF-1 and TGF-β1 in the Formation of Rat Skeletal Muscle Fibrosis. China Sport Science, 31(11): 61-70. DOI: 10.16469/j.css.2011.11.012
	XIAO Wei-hua, CHEN Pei-jie. 2011: Overtraining Inhibiting Phagocytosis of Rat Peritoneal Macrophages:Role of IGF-1 and MGF. China Sport Science, 31(10): 67-71,78. DOI: 10.16469/j.css.2011.10.009
	CHEN Tao, CHEN Cai-zhen, LU Jian. 2011: The Effects of Endurance,Strength and Mixing Type Training on Satellite Cell Activation Signaling Pathway in Adult C57BL /6 Mouse Skeletal Muscle. China Sport Science, 31(9): 69-76. DOI: 10.16469/j.css.2011.09.009
	CHEN Ying-jie, ZHAO Guang-gao. 2011: NO Effects of Transcriptional the Regulation of Rat Gastrocnemius Mitochondria Biosynthesis after a High-load Exercise. China Sport Science, 31(3): 58-62,67. DOI: 10.16469/j.css.2011.03.011
	XIAO Wei-hua, CHEN Pei-jie, WANG Ru, DONG Jing-mei. 2011: Effects of Overtraining and Supplement with DPI or Glutamine on the Production of ROS and the Expression of iNOS in Pritoneal Macrophages. China Sport Science, 31(2): 49-54. DOI: 10.16469/j.css.2011.02.003

Effect and Mechanism of Blood Flow Restriction Combined with Low Load Training on Blood Pressure of Male Spontaneously Hypertensive Rats